Structural Insight into an Enzyme that Breaks Down Oligosaccharides in Caramel

Insight into an implicit time integration scheme for structural dynamics

0045-7949/$ see front matter 2012 Elsevier Ltd. A doi:10.1016/j.compstruc.2012.01.009 ⇑ Corresponding author. E-mail address: [email protected] (K.J. Bathe). In Refs. [1,2], an effective implicit time integration scheme was proposed for the finite element solution of nonlinear problems in structural dynamics. Various important attributes were demonstrated. In particular, it was shown that the scheme ...

Structural insight into African horsesickness virus infection.

African horsesickness (AHS) is a devastating disease of horses. The disease is caused by the double-stranded RNA-containing African horsesickness virus (AHSV). Using electron cryomicroscopy and three-dimensional image reconstruction, we determined the architecture of an AHSV serotype 4 (AHSV-4) reference strain. The structure revealed triple-layered AHS virions enclosing the segmented genome an...

Structural Insight into RNA Hairpin Folding Intermediates

Hairpins are a ubiquitous secondary structure motif in RNA molecules. Despite their simple structure, there is some debate over whether they fold in a two-state or multi-state manner. We have studied the folding of a small tetraloop hairpin using a serial version of replica exchange molecular dynamics on a distributed computing environment. On the basis of these simulations, we have identified ...

Structural insight into TPX2-stimulated microtubule assembly

During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The molecular mechanism of how TPX2 stimulates MT assembly remains unknown because structural information about the interaction of TPX2 with MTs is lacking. Here, we determine the cryo-electron microsco...

Structural Insight into Golgi Membrane Stacking